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KMID : 0364219840270030127
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Korean Journal of Zoology
1984 Volume.27 No. 3 p.127 ~ p.136
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Effect of Gravity Perturbation on the Axis Specification in the Egg of Xenopus laevis
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Chung Hae-Moon
Han Pyeong-Lim
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Abstract
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The specification of dorsal/ventral axis in the egg of Xenopus laevis was investigated as a series of oblique orientation to gravity by tilt and clinostat. The results are as follows. (1) If the eggs were oriented, in the early period after fertilization, to novel gravity by $15^\\circ, 30^\\circ, 45^\\circ$ and $60^\\circ$ tilt until gastrula stage, the site of involution was usually formed in the OpG side (the side opposing gravity). As the degree of tilt was raised from $15^\\circ to 60^\\circ$, the rate of relocation of the involution site was proportionally increased. (2) When UV-irradiated eggs were tilted period to first cleavage by $15^\\circ, 30^\\circ, 45^\\circ$ and $6\^\\circ$, the effect of UV syndrome was rescued, and the extent of rescue was propotional to the tilt degree. (3) The fertile eggs were loaded on the clinostats of several speeds. In the range of low speeds between $0.45 \\sim 9.0$ rph, the location of dorsal lip was dependent on the direction of rotation, and in $40 \\sim 360$ rph, lip was formed at a random position. In addition, some of the tadpoles experienced with clinostat showed the typical syndrome of "dorsal axis reduction". The above results were discussed regarding the mechanism of the establishment of dorsal/ventral palarity. palarity.
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